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| Freitas,A.K.L.; Silva,M.T.B.; Silva,C.M.S.; Prata,M.M.G.; Rodrigues,F.A.P.; Siqueira,R.J.B.; Lima,A.A.M.; Santos,A.A.; Havt,A.. |
| Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive training protocol alter intestinal permeability and the putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 and 3) trained (50 min per day, 5 days per week for 12 weeks) with or without 6 weeks oral (1.5 g/kg) AG supplementation; 4 and 5) trained and subjected to an additional exhaustive test protocol with or without oral AG supplementation. Venous blood samples were collected to determine gasometrical indices at the end of the 12-week protocol or after exhaustive test. Lactate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Claudin-2; Intestinal permeability; Lactulose-mannitol test; Physical exercise; Tight junctions. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500602 |
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| Alves,N.T.Q.; Ximenes,R.M.; Jorge,R.J.B.; Silveira,J.A.M.; Santos,J.V.A.; Rodrigues,F.A.P.; Costa,P.H.S.; Xavier Jr.,F.A.F.; Evangelista,J.S.A.M.; Havt,A.; Soares,V.C.G.; Toyama,M.H.; Oliveira,A.N.A.; Araújo,R.M.; Alves,R.S.; Monteiro,H.S.A.. |
| Bredemeyera floribunda roots are popularly used to treat snakebites in the semiarid region of Northeast Brazil, and previous studies indicate the anti-ophidian actions of triterpenoid saponins found in its roots. To assess B. floribunda root extract (BFRE) activity against the effects of Bothrops jararacussu venom (BjuV), antiphospholipasic, antiproteolytic, antihemorrhagic, antinecrotic, and anti-edematogenic activities were investigated in mice. Phytochemical analysis revealed the presence of saponins, flavonoids, and sugars, with rutin and saccharose being the major constituents of BFRE. Acute toxicity was determined and BFRE was nontoxic to mice. Phospholipase A2 and proteolytic activities induced by BjuV were inhibited in vitro by BFRE at all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bredemeyera floribunda; Snakeroot; Snake venom; Rutin; Antihemorrhagic; Anti-edematogenic. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100608 |
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| Sampaio,I.C.; Medeiros,P.H.Q.S.; Rodrigues,F.A.P.; Cavalcante,P.A.; Ribeiro,S.A.; Oliveira,J.S.; Prata,M.M.G.; Costa,D.V.S.; Fonseca,S.G.C.; Guedes,M.M.; Soares,A.M.; Brito,G.A.C.; Havt,A.; Moore,S.R.; Lima,A.A.M.. |
| Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Undernutrition; Ion transport; Intestinal absorption. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607 |
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| Cavalcante,P.A.; Prata,M.M.G.; Medeiros,P.H.Q.S.; Alves da Silva,A.V.; Quetz,J.S.; Reyes,M.A.V.; Rodrigues,T.S.; Santos,A.K.S.; Ribeiro,S.A.; Veras,H.N.; Bona,M.D.; Amaral,M.S.M.G.; Rodrigues,F.A.P.; Lima,I.F.N.; Havt,A.; Lima,A.A.M.. |
| Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic Escherichia coli; Intestinal cell migration; Type III secretion system. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602 |
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